EN 14476 Test | An Antiviral Textile Test

What is the EN 14476 test?  

  • The test method of EN 14476 is used for the quantitative estimation of an antiviral textile test that measures viral antimicrobial activity for disinfectants and sanitizers.  
  • The test method of EN 14476 is a virucidal activity of chemical disinfectant and antiseptic products. The test forms a homogenous stable preparation when diluted with hot water or in the case of ready-to-use products. Products will not get diluted when it is applied with water.  

What is the process of the EN 14476 test?  

Here is the process of how an EN 14476 test is done.   

  1. A sample is prepared in test tube A with 1 ml of interfering substance and 8 ml of customer product. 
  2. Then, 1 ml of virus suspension is injected into test tube A. 
  3. Now it is the contact time, which is incubation. Specific time and temperatures should be fixed for contact 
  4. Next step is neutralization. After the contact period, the test product is neutralized by adding a test tube mixture into the ice-cold medium. 
  5. Serial dilutions of the virus should be prepared. 
  6. Inject cells into 96 wells and grow to confluency. After you receive the required confluency add 100ul of a virus. 
  7. Now add ten-fold serial dilutions of the virus to cells in a multiple-well plate. 
  8. The cells will be examined in each well for the CPE. 
  9. Then, the result will be displayed. 

The products are been tested only at a particular concentration that is of 80% as some dilution is always produced by including the test organisms and interfering substances. There are some exceptional cases where the products are been tested at a concentration of 97% with the different method. 

As part of deciding the correct scope for antiviral testing of a product, the category known for the product application is used to calculate the exposure time and test conditions for validating the antiviral performance of the product. There are four product application categories. They are as follows:  

Generally, this test is done to the products that are frequently used in medica areas. The products that are been tested includes a handwash, hand sanitizer, disinfectant spray, surface disinfectant and textile disinfection. The test is done if the particular area is medically suspected with the disinfection. 

What are the places that are generally suspected of the EN 14476 test?  

Generally, suspicious situations may occur in such areas. They are:  

  • They occur in health care organizations like dental institutions, hospitals and community medical facilities.  
  • It may happen in clinics of schools, nursing homes, and kindergartens.  
  • It also may appear in work location and the house.  
  • The other services may also include laundries and kitchens where they supply products directly to the patients.  

The exposure time may vary from half a minute to one hour for the different product categories. Adding the exposure time, testing can include hard water dilutions, soiling, and a range of different virus types.  

In this EN 14476 test, several virus types can be tested, whereas common types include influenza and coronavirus strains. The correct performance of the treated textile is known by viral titer measurements using a plaque test or TCID50 method. TCID50 is the Median Tissue Culture Infectious Dose parallel to the possible number serial dilution measurement.  

What are the organisms that are been used for the EN 14476 test?  

  1. SARS CoV-2 
  2. Beta coronavirus (43) (ATCC VR-1558) 
  3. Human coronavirus (229E) (ATCC VR-740) 
  4. Influenza A (H1N1) (ATCC VR-1469) Influenza A (H3N2) (ATCC VR-1679) 
  5. Poliovirus 
  6. Adenovirus 
  7. Murine norovirus 

The EN 14476  

Common Antimicrobial Additives  

In this EN 14476 test, it requires some standard mechanisms that should change some features of the virus both chemically and dynamically. The strategy of the drug is dependent on particular binding to the virus which is known as receptor ligand-binding. The receptor tends to be virus and the ligand tends to be the drug which is used to cover to the receptor by stopping the mode of action. 

For other products, the usual approach is to fight with a more general features of the virus chemically. The coronavirus has a substance that is surfaced out with lipid; the antiviral products will stick to the membrane and disturb it, which leads to losing its original shape and, therefore, its ability to infect.  

There are some standard classes of antimicrobials that are used for the activity. They are:  

  • Reactive aldehydes  
  • Oxides such as hydrogen peroxide or peroxyacetic acid  
  • Quaternary ammonium compounds  
  • Essential oils  
  • Quaternary-silane molecules  

Liquid Disinfectant:  

For this liquid disinfectant application, importance is given for a fast action rate. Some favored compounds commonly use aldehyde chemistries, such as formaldehyde and glutaraldehyde, peroxides, hypochlorite, and to some extent, quaternary ammonium compounds. The very most widely used combinations of compounds are peroxides and quaternary ammonium compounds, as they can collaboratively perform antiviral actions. They provide swift action with high antiviral efficacy at lower antimicrobial additive concentrations.  

Most people request for durability testing with EN 14476 to get awareness about the antimicrobial performance with viruses when exposed to environmental conditions. There are some frequently used forms for durability testing that include some conditions which are known as interfering substances and other substances such as serum, blood, or hard water.  

Five control tests run simultaneously to eliminate other possible explanations for the test results for the best efficacy result. Take a look:  

  1. Virus control 
  2. Cytotoxicity control 
  3. Suppression control 
  4. Interference control 
  5. Reference control 


Do you want to test your sanitizers or disinfectants whether they are working well or not? It is the right choice to go with an EN 14476 test available at Microbe Investigations AG.  

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